InStab ™ Phosphatase Inhibitor Cocktail(100×,Stock Solution)

Mfr.No.

20109ES, 20109ES05, 20109ES20, 20109ES70

Description

The phosphorylation and dephosphorylation of proteins in cells are regulatory switches for many important biological activities such as signal transduction, cell proliferation, cell differentiation, and apoptosis. Therefore, controlling the action of phosphatases is of significant physiological importance in protein research. Tissues and cells are filled with a large number of proenzymes, which are fully activated after lysis and begin to digest surrounding proteins. Therefore, protecting the phosphorylation state of proteins is extremely critical in the study of protein phosphorylation pathways and signal transduction.

This product is a special mixture of multiple phosphatase inhibitors, which broadly inhibits non-specific acidic/alkaline phosphatases as well as specific protein tyrosine phosphatases, serine/threonine protein phosphatases (PPA, PP2A), etc.

The product is provided in the form of A and B tubes, and the two solutions can target different types of phosphatases to exert synergistic inhibitory effects.

Tube A contains Cantharidin, Bromotetramisole, and Microcystin-LR, provided as a ready-to-use solution in DMSO, inhibiting the L-isozyme of alkaline phosphatase and serine/threonine protein phosphatases, such as protein phosphatase 1 and 2A (PP1 and PP2A). Tube B contains Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole, and Sodium Fluoride, provided as a ready-to-use solution in ddH2O, inhibiting tyrosine protein phosphatases, acidic phosphatases, and alkaline phosphatases.

This product can be used for WB, Co-IP, Pull-down assay, IF, IHC, kinase research, and various other analyses.

Kit Components

Components No.	Name	20109ES05	20109ES20	20109ES70
20109	InStab ™ Phosphatase Inhibitor Cocktail(100×,Stock Solution)	1 mL	10×1 mL	100×1 mL

Notes

Instructions

Before use, take the product out of the refrigerator and allow it to warm to room temperature. Add A solution and B solution to the sample at a ratio of 1:100 (v/v), and mix thoroughly after each addition.

1. The solvent for Tube A is DMSO, and for Tube B is ddH2O. DMSO dissolves more slowly, so it takes longer to completely dissolve A solution compared to B solution. At lower temperatures, A solution can be placed in a 25℃ water bath for dissolution.

2. Mixing A and B solutions before use can easily form precipitates, so this method should be avoided. It is recommended to add appropriate amounts of A solution and B solution separately to the sample.

3. This product can tolerate 1% SDS, Triton, NP-40.

Notes

1. It is recommended to aliquot and freeze the 100× stock solution to avoid repeated freeze-thaw cycles. Working solutions should be prepared fresh.

2. This product does not contain protease inhibitors. Appropriate protease inhibitors can be added to the sample to be analyzed according to experimental needs.

3. The solvent for Tube A is DMSO, please handle with care.

4. DTT, EDTA, EGTA, etc., may affect the sodium orthovanadate contained in this product, so handle with care..

5. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！

Shipping

The product is shipped with ice pack.

Storage	Stored at -15~-25℃ for 1 year, or stored at 2~8℃ for 2 months.

Product Manual	Safety Data Sheet 20109-A-MSDS-CN20250110 20109-B-MSDS-CN20250110 Manuals 20109_Manual_CN20250107_EN

Reference	[1] Zhu R, Yan T, Feng Y, et al. Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms. Cell Res. 2021;31(12):1244-1262. doi:10.1038/s41422-021-00573-y(IF:25.617) [2] Liu H, Zhou Y, Qiu H, et al. Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src. Cell Death Dis. 2021;12(4):284. Published 2021 Mar 17. doi:10.1038/s41419-021-03561-7(IF:8.469) [3] Chen Z, Xie J, Li Q, et al. Human CYP enzyme-activated clastogenicity of 2-ethylhexyl diphenyl phosphate (a flame retardant) in mammalian cells. Environ Pollut. 2021;285:117527. doi:10.1016/j.envpol.2021.117527(IF:8.071) [4] Sun J, Guo Y, Fan Y, Wang Q, Zhang Q, Lai D. Decreased expression of IDH1 by chronic unpredictable stress suppresses proliferation and accelerates senescence of granulosa cells through ROS activated MAPK signaling pathways. Free Radic Biol Med. 2021;169:122-136. doi:10.1016/j.freeradbiomed.2021.04.016(IF:7.376) [5] Yang BY, Deng GY, Zhao RZ, et al. Porous Se@SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-κB pathway inhibition. Acta Biomater. 2019;88:392-405. doi:10.1016/j.actbio.2019.02.006(IF:6.638) [6] Zhang Y, Li G, Zhang X, Lin L. ROS-scavenging glyco-nanoplatform for synergistic antibacterial and wound-healing therapy of bacterial keratitis. J Mater Chem B. 2022;10(24):4575-4587. Published 2022 Jun 22. doi:10.1039/d2tb00667g(IF:6.331) [7] Deng L, Li H, Su X, et al. Chlorzoxazone, a small molecule drug, augments immunosuppressive capacity of mesenchymal stem cells via modulation of FOXO3 phosphorylation. Cell Death Dis. 2020;11(3):158. Published 2020 Mar 2. doi:10.1038/s41419-020-2357-8(IF:6.304) [8] Zhang X, Cai Z, Wu M, Huangfu X, Li J, Liu X. Adipose Stem Cell-Derived Exosomes Recover Impaired Matrix Metabolism of To Human Rotator Cuff Tendons by Maintaining Tissue Homeostasis. Am J Sports Med. 2021;49(4):899-908. doi:10.1177/0363546521992469(IF:6.203) [9] Gao C, Wang SW, Lu JC, et al. KSR2-14-3-3ζ complex serves as a biomarker and potential therapeutic target in sorafenib-resistant hepatocellular carcinoma. Biomark Res. 2022;10(1):25. Published 2022 Apr 25. doi:10.1186/s40364-022-00361-9(IF:6.148) [10] Chen L, Xu W, Yang Q, et al. Imperatorin alleviates cancer cachexia and prevents muscle wasting via directly inhibiting STAT3. Pharmacol Res. 2020;158:104871. doi:10.1016/j.phrs.2020.104871(IF:5.893)

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Combination SKU	Supplier No.	Size	Your price
BHB20800027-1	20109ES05	2ml	$45	Add to Cart
BHB20800027-2	20109ES20	10x2mL	$270	Add to Cart
BHB20800027-3	20109ES70	100x2mL	$1715	Add to Cart

InStab ™ Phosphatase Inhibitor Cocktail(100×,Stock Solution)

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