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Immortalized Human Mammary Fibroblasts (HMF3S)

SKU : BHC10900581

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Applied Biological Materials (abm) Inc.

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Description
Review(s)

Mfr.No.	T0154
Description	Immortalized Human Mammary MUC+ESA+ Epithelial Cells are luminal-derived epithelial cells coexpressing sialomucin (MUC) and epithelial-specific antigen (ESA). It has tight junction proteins, occludin, claudin-1, and the ability to build high transepithelial electrical resistance which is all characteristics of differentiated luminal epithelial phenotypes. It can form acinus-like spheres when cultures on laminin rich gel. These cells have characteristics of differentiated mature human breasts.
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level	CK18, E-cadherin
Growth Properties	Adherent
Quantity	1x106 cells / 1.0 ml
Tissue	Breast
Morphology	Polygonal
Population Doubling	36 - 46 hours
Species	Human (H. sapiens)
Propagation	Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow III medium:Prigrow IV medium (3:1 ratio) available at abm, Cat. No.'s TM003 and TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, hydrocortisone to a final concentration of 0.5 µg/ml, Gentamicin (G272) to a final concentration of 50 µg/ml, Insulin Solution (TM053) to a final concentration of 5 µg/ml, Recombinant Human EGF (Z100139) to a final concentration of 10 ng/ml, cholera toxin to a final concentration of 10-10 M, adenine to a final concentration of 1.8 x 10-4 M, Y27632 to a final concentration of 10 µM, and L-glutamine (G275) to a final concentration of 2 mM.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control	1) Immunocytochemical staining

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SKU	Supplier No.	Your price	Quantity
BHC10900581	T0154	$3950		Add to Cart

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