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Home / Proteins & Peptide

IL-6, Mouse

SKU : BHP18500058

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iXCells Biotechnologies

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Mfr.No.	IX02767, IX02767-1, IX02767-10
Description	Interleukin 6 is a multi functional protein that plays important roles in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts, hepatocytes, keratinocytes, astrocytes, vascular endothelial cells, and various tumor cells. The production of IL-6 is upregulated by numerous signals including mitogenic or antigenic stimulation, LPS, calcium ionophore, IL-1, IL-2, IFN, TNF, PDGF, and viruses. IL-6 expression in monocytes is inhibited by IL-4 and IL-13.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20 °C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Molecular Weight	Approximately 21.7 kDa, a single non-glycosylated polypeptide chain containing 188 amino acids.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	Mouse
Endotoxin Level	Less than 1 EU/ug of rMuIL-6 as determined by LAL method.
Bioactivity	Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of 5 x 107 IU/mg.

Synonyms	Interleukin-6 (IL-6), Mouse;
Sequence	MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIVEMRKELCNGN?SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKISSGLLEYHSY?LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKIVLPTPISNAL?LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

Purity	97 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500058-1	IX02767-1	1mg	$3775		Add to Cart
BHP18500058-2	IX02767-10	10ug	$199		Add to Cart

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