The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.Mark H. L. Lambermon, Mol. Cell. Biol., Jun 2002; 22: 4346 - 4357. Kyoung-Seok Ryu, J. Biol. Chem., Sep 2003; 278: 36621 - 36627. R. Jrgen Dohmen, J. Biol. Chem., Jul 1995; 270: 18099. Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096.
Host
Rabbit
Immunogen
Synthesized peptide derived from C-terminal of Human Uba2.
Raised In
Rabbit
Reactivity
Human
Regulatory
RUO
Relevance
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Mark H. L. Lambermon, Mol. Cell. Biol., Jun 2002; 22: 4346 - 4357. Kyoung-Seok Ryu, J. Biol. Chem., Sep 2003; 278: 36621 - 36627. R. Jrgen Dohmen, J. Biol. Chem., Jul 1995; 270: 18099. Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of total Uba2 protein.
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Pathway
Ubiquitin mediated proteolysis
Protein Families
Ubiquitin-activating E1 family
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.