JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3
Antigen
Smad3
Categories
Primary Antibodies
Clonality
polyclonal
Description
Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. Shi W, et al. J Cell Sci. 2007 Apr 1;120(Pt 7):1216-24Seong HA, et al. J Biol Chem. 2007 Apr 20;282(16):12272-89Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200LeClair RJ, et al. Circ Res. 2007 Mar 30;100(6):826-33
Host
Rabbit
Immunogen
Peptide sequence around aa.423~427 (C-S-S-V-S) derived from Human Smad3.
Involvement In Disease
Colorectal cancer (CRC); Loeys-Dietz syndrome 3 (LDS3)
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Shi W, et al. J Cell Sci. 2007 Apr 1;120(Pt 7):1216-24 Seong HA, et al. J Biol Chem. 2007 Apr 20;282(16):12272-89 Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200 LeClair RJ, et al. Circ Res. 2007 Mar 30;100(6):826-33
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of total Smad3 protein.
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.