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Home / Primary Antibodies / IHC Antibodies

RB1 (Ab-807) Antibody

SKU : BHA10558982

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA445358
Alternative Names	P105-RB; PP105; PP110; RB-1; RB1
Antigen	RB1
Categories	Primary Antibodies
Clonality	polyclonal
Description	Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.
Host	Rabbit
Immunogen	Peptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb.
Involvement In Disease	Childhood cancer retinoblastoma (RB); Bladder cancer (BLC); Osteogenic sarcoma (OSRC)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572. Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776. Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783. Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total Rb protein.
Subcellular Location	Nucleus

Uniprot	P06400
Database Links	HGNC:9884 OMIM:109800 KEGG:hsa:5925 STRING:9606.ENSP00000267163 UniGene:Hs.408528
Function	Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Pathway	Cell cycle Cellular senescence
Protein Families	Retinoblastoma protein (RB) family
Tissue Specificity	Expressed in the retina.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:1000
IHC	1:50-1:200

Tested Application

ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10558982	CSB-PA445358	100ul	$297		Add to Cart

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