Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.Chong H, et al. (2001) EMBO J. 20, 3716-3727.King A J, et al. (1998) Nature. 396:180-183.Fabian J R, et al. (1993) Mol Cell Biol. 13: 7170-7179.
Host
Rabbit
Immunogen
Peptide sequence around aa.257~261 (S-T-S-T-P) derived from Human RAF.
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224. Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468. Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183. Fabian J R, et al. (1993) Mol Cell Biol. 13: 7170-7179.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of total Raf1 protein.
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases
Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
Tissue Specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2.
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.