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Home / Primary Antibodies / IHC Antibodies

PXN (Ab-31) Antibody

SKU : BHA10559020

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA944033
Alternative Names	PAXI; PXN;
Categories	Primary Antibodies
Clonality	polyclonal
Description	An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions Davidson D, et al. (2001) EMBO J; 20(13): 3414-3426 Kook S, et al. (2000) Mol Biol Cell; 11(3): 929-939 Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96(3): 1123-1128 Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65(4): 595-626 Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96(15): 8449-8454
Host	Rabbit
Immunogen	Peptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Human Paxillin.
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions Davidson D, et al. (2001) EMBO J; 20(13): 3414-3426 Kook S, et al. (2000) Mol Biol Cell; 11(3): 929-939 Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96(3): 1123-1128 Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65(4): 595-626 Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96(15): 8449-8454
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total Paxillin protein.
Subcellular Location	Cytoplasm, cytoskeleton, Cell junction, focal adhesion, Cytoplasm, cell cortex

Uniprot	P49023
Database Links	HGNC:9718 OMIM:602505 KEGG:hsa:5829 STRING:9606.ENSP00000228307 UniGene:Hs.446336
Function	Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Pathway	Chemokine signaling pathway VEGF signaling pathway Focal adhesion Regulation of actin cytoskeleton Leukocyte transendothelial migration
Protein Families	Paxillin family

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:1000
IHC	1:50-1:200

Tested Application

ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559020	CSB-PA944033	100ul	$297		Add to Cart

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