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Home / Primary Antibodies / IHC Antibodies

Phospho-ZAP70 (Tyr319) Antibody

SKU : BHA10560549

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA969675
Alternative Names	SRK, STD, TZK, STCD, ZAP-70
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448 Ku GM, et al. (2001) EMBO J; 20(3): 457-465 Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780 Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774 Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844 Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y(p)-S-D) derived from Human Zap-70.
Involvement In Disease	Immunodeficiency 48 (IMD48); Autoimmune disease, multisystem, infantile-onset, 2 (ADMIO2)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448 Ku GM, et al. (2001) EMBO J; 20(3): 457-465 Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780 Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774 Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844 Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 1214.
Subcellular Location	Cytoplasm, Cell membrane, Peripheral membrane protein

Uniprot	P43403
Database Links	HGNC:12858 OMIM:176947 KEGG:hsa:7535 STRING:9606.ENSP00000264972 UniGene:Hs.234569
Function	Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins
Pathway	NF-kappa B signaling pathway Ras signaling pathway Natural killer cell mediated cytotoxicity T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation
Protein Families	Protein kinase superfamily, Tyr protein kinase family, SYK/ZAP-70 subfamily
Tissue Specificity	Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC, IF

Description

ELISA ; WB ; IHC ; IF, WB ; ELISA ; IHC ; IF

Dilution

WB	1:500-1:1000
IHC	1:50-1:100
IF	1:100-1:200

Tested Application

ELISA,WB,IHC,IF;WB:1:500-1:1000,IHC:1:50-1:100,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560549	CSB-PA969675	100ul	$360		Add to Cart

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