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Home / Primary Antibodies / IHC Antibodies

Phospho-RASGRF1 (Ser916) Antibody

SKU : BHA10560165

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA256747
Alternative Names	CDC25; GNRP; GRF1; RASGRF1;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285. Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189. Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379. Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1.
Raised In	Rabbit
Reactivity	Mouse, Rat
Regulatory	RUO
Relevance	Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity. Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285. Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189. Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379. Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.

Uniprot	Q13972
Database Links	HGNC:9875 OMIM:606600 KEGG:hsa:5923 STRING:9606.ENSP00000405963 UniGene:Hs.459035
Function	Promotes the exchange of Ras-bound GDP by GTP.
Pathway	MAPK signaling pathway Focal adhesion MAPK Erk pathway Ras signaling pathway Excitatory synapse pathway

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, IHC

Description

ELISA ; IHC, IHC ; ELISA

Dilution

IHC	1:50-1:100

Tested Application

ELISA,IHC;IHC:1:50-1:100

Datasheet
ELISA Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560165	CSB-PA256747	100ul	$360		Add to Cart

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