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Home / Primary Antibodies / IHC Antibodies

Phospho-PKMYT1 (Ser83) Antibody

SKU : BHA10560192

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA729561
Alternative Names	PMYT1; PKMYT1; MYT1 kinase;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.Booher R.N., J. Biol. Chem. 272:22300-22306(1997).Liu F., Mol. Cell. Biol. 17:571-583(1997).Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of Serine 83(R-V-S(p)-F-R) derived from Human MYT1.
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Booher R.N., J. Biol. Chem. 272:22300-22306(1997). Liu F., Mol. Cell. Biol. 17:571-583(1997). Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of MYT1 only when phosphorylated at serine 83.
Subcellular Location	Endoplasmic reticulum membrane, Peripheral membrane protein, Golgi apparatus membrane, Peripheral membrane protein

Uniprot	Q99640
Database Links	HGNC:29650 OMIM:602474 KEGG:hsa:9088 STRING:9606.ENSP00000262300 UniGene:Hs.732385
Function	Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
Pathway	Cell cycle
Protein Families	Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, IHC

Description

ELISA ; IHC, IHC ; ELISA

Dilution

IHC	1:50-1:100

Tested Application

ELISA,IHC;IHC:1:50-1:100

Datasheet
ELISA Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560192	CSB-PA729561	100ul	$360		Add to Cart

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