PDPK1; PkB kinase; Protein kinase B kinase; hPDK1; kinase PDK1
Categories
Primary Antibodies ; Phospho Antibodies
Clonality
polyclonal
Description
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63Chen H, et al. (2001) Biochemistry; 40(39): 11851-9Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1.
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.
Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63 Chen H, et al. (2001) Biochemistry; 40(39): 11851-9 Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7 Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of PDK1 only when phosphorylated at serine 241.
Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include
Protein kinase superfamily, AGC Ser/Thr protein kinase family, PDPK1 subfamily
Tissue Specificity
Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.