Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.Geller D.A., Proc. Natl. Acad. Sci. U.S.A. 90:3491-3495(1993).Sherman P.A., Biochemistry 32:11600-11605(1993).Charles I.G., Proc. Natl. Acad. Sci. U.S.A. 90:11419-11423(1993).
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of tyrosine 151(Q-Y-Y(p)-G-S) derived from Human iNOS .
Raised In
Rabbit
Reactivity
Human, Mouse
Regulatory
RUO
Relevance
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Geller D.A., Proc. Natl. Acad. Sci. U.S.A. 90:3491-3495(1993). Sherman P.A., Biochemistry 32:11600-11605(1993). Charles I.G., Proc. Natl. Acad. Sci. U.S.A. 90:11419-11423(1993).
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of Inos only when phosphorylated at tyrosine 151.
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.