Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.Soosairajah J, et al. (2005) EMBO J.Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2.
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of LIMK2 only when phosphorylated at threonine 505.
Subcellular Location
Isoform LIMK2a: Cytoplasm, Nucleus, Note=Isoform LIMK2a is distributed in the cytoplasm and the nucleus, SUBCELLULAR LOCATION: Isoform LIMK2b: Cytoplasm, Nucleus
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Pathway
Regulation of actin cytoskeleton Fc gamma R-mediated phagocytosis
Protein Families
Protein kinase superfamily, TKL Ser/Thr protein kinase family
Tissue Specificity
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung,
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.