KC1A; CKI-alpha; CSNK1A1; alpha isoform; Casein kinase I
Categories
Primary Antibodies ; Phospho Antibodies
Clonality
polyclonal
Description
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.Tapia C., FEBS Lett. 349:307-312(1994).Fish K.J., J. Biol. Chem. 270:14875-14883(1995).Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α.
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.Tapia C., FEBS Lett. 349:307-312(1994).Fish K.J., J. Biol. Chem. 270:14875-14883(1995).Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of CK-1α only when phosphorylated at tyrosine 294.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
ELISA, WB, IHC
Description
ELISA ; WB ; IHC, WB ; ELISA ; IHC
Dilution
WB
1:500-1:1000
IHC
1:50-1:100
Tested Application
ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:100
Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Reviews of Phospho-CSNK1A1/CSNK1A1L (Tyr294) Antibody