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Home / Primary Antibodies / IHC Antibodies

Phospho-CFL1 (Ser3) Antibody

SKU : BHA10560545

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA932779
Alternative Names	CFL; CFL1;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.Wang Y, et al. (2005) Biol Chem 280(13): 12683-9.Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Kobayashi M, et al. (2006) EMBO J 25(4): 713-26. Wang Y, et al. (2005) Biol Chem 280(13): 12683-9. Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
Subcellular Location	Nucleus matrix, Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, lamellipodium membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, lamellipodium

Uniprot	P23528
Database Links	HGNC:1874 OMIM:601442 KEGG:hsa:1072 STRING:9606.ENSP00000309629 UniGene:Hs.170622
Function	Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation
Pathway	Regulation of actin cytoskeleton Fc gamma R-mediated phagocytosis
Protein Families	Actin-binding proteins ADF family
Tissue Specificity	Widely distributed in various tissues.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC, IF

Description

ELISA ; WB ; IHC ; IF, WB ; ELISA ; IHC ; IF

Dilution

WB	1:500-1:1000
IHC	1:50-1:100
IF	1:100-1:200

Tested Application

ELISA,WB,IHC,IF;WB:1:500-1:1000,IHC:1:50-1:100,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560545	CSB-PA932779	100ul	$360		Add to Cart

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