Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46Le XF, et al. (2003) J Biol Chem;Connor MK, et al. (2003)
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1.
Involvement In Disease
Multiple endocrine neoplasia 4 (MEN4)
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.
Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46 Le XF, et al. (2003) J Biol Chem; Connor MK, et al. (2003)
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.
Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.