RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3
Antigen
BLM
Categories
Primary Antibodies ; Phospho Antibodies
Clonality
polyclonal
Description
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).Ellis N.A., Cell 83:655-666(1995).Karow J.K., J. Biol. Chem. 272:30611-30614(1997).Pichierri P., EMBO J. 23:3154-3163(2004).
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.
Involvement In Disease
Bloom syndrome (BLM)
Raised In
Rabbit
Reactivity
Human
Regulatory
RUO
Relevance
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Ellis N.A., Cell 83:655-666(1995). Karow J.K., J. Biol. Chem. 272:30611-30614(1997). Pichierri P., EMBO J. 23:3154-3163(2004).
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.
ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction
Protein Families
Helicase family, RecQ subfamily
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.