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Home / Primary Antibodies / IHC Antibodies

PCNA Antibody

SKU : BHA10559766

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA974334
Alternative Names	cyclin; proliferating cell nuclear antigen;
Categories	Primary Antibodies
Clonality	polyclonal
Description	Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.Anje Sporbert, Nucleic Acids Res., Jun 2005; 33: 3521 - 3528. Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007; 292: L716 - L724. Basavaraj Bagewadi, J. Virol., Nov 2004; 78: 11890 - 11903. T Iida, I Suetake, Genes Cells, Oct 2002; 7: 997 -
Host	Rabbit
Immunogen	Synthesized peptide derived from Human PCNA.
Involvement In Disease	Ataxia-telangiectasia-like disorder 2 (ATLD2)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. Anje Sporbert, Nucleic Acids Res., Jun 2005; 33: 3521 - 3528. Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007; 292: L716 - L724. Basavaraj Bagewadi, J. Virol., Nov 2004; 78: 11890 - 11903. T Iida, I Suetake, Genes Cells, Oct 2002; 7: 997 -
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total PCNA protein.
Subcellular Location	Nucleus

Uniprot	P12004
Database Links	HGNC:8729 OMIM:176740 KEGG:hsa:5111 STRING:9606.ENSP00000368438 UniGene:Hs.147433
Function	Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways
Pathway	Cell cycle DNA repair pathway
Protein Families	PCNA family

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:3000
IHC	1:50-1:100

Tested Application

ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559766	CSB-PA974334	100ul	$297		Add to Cart

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