CDC46; DNA replication licensing factor MCM5; minichromosome maintenance deficient protein 5;
Antigen
MCM5
Categories
Primary Antibodies
Clonality
polyclonal
Description
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity. Interacts with MCMBP.Robert A. Sclafani, Genetics, May 2002; 161: 47. Marylynn Snyder, PNAS, Oct 2005; 102: 14539 - 14544. Christopher J. DaFonseca, PNAS, Mar 2001; 98: 3034. Cathleen M. Lake, Genetics, Jun 2007; 10.1534
Host
Rabbit
Immunogen
Synthesized peptide derived from Human MCM5 antibody.
Involvement In Disease
Meier-Gorlin syndrome 8 (MGORS8)
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity. Interacts with MCMBP.
Robert A. Sclafani, Genetics, May 2002; 161: 47. Marylynn Snyder, PNAS, Oct 2005; 102: 14539 - 14544. Christopher J. DaFonseca, PNAS, Mar 2001; 98: 3034. Cathleen M. Lake, Genetics, Jun 2007; 10.1534
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of total MCM5 protein.
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.
Pathway
Cell cycle
Protein Families
MCM family
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.