mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587?nm and 610?nm respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour as well as its photostability compared to other monomeric fluorophores.
Host
Mouse
Raised In
Mouse
Regulatory
RUO
Relevance
mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.
Specificity
The mCherry tag antibody can recognize mCherry and mCherry tag fusion proteins.
Buffer
pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.al
Form
Liquid
Format
Liquid
Purification
Antigen Affinity Purified
Purity
Antigen Affinity Purified
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
WB, IHC, IP, IF, ELISA
Description
WB ; IHC ; IP ; IF ; ELISA, IP ; WB ; IHC ; IF
Dilution
WB
1:500-1:2000
IHC
1:100-1:300
IP
2-5μg/mg
IF
1:200-1:1000
ELISA
1:20000
Tested Application
WB, IHC, IF, IP; Recommended dilution: Western blotting: 1:5000~1:10000