The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. In cell and molecular biology the GFP gene is frequently used as a reporter of expression.
Host
Mouse
Raised In
Mouse
Regulatory
RUO
Relevance
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
Specificity
The GFP tag antibody can recognize GFP, EGFP and GFP, EGFP tag fusion proteins. It may also recognize RFP, YFP, CFP.
Buffer
pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.al
Form
Liquid
Format
Liquid
Purification
Antigen Affinity Purified
Purity
Antigen Affinity Purified
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
WB, IHC, IP, IF, ELISA
Description
WB ; IHC ; IP ; IF ; ELISA, IP ; WB ; IHC ; IF
Dilution
WB
1:500-1:2000
IHC
1:100-1:300
IP
2-5μg/mg
IF
1:200-1:1000
ELISA
1:20000
Tested Application
WB, IHC, IF, IP; Recommended dilution: Western blotting: 1:5000~1:10000