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Home / Primary Antibodies / IHC Antibodies

EP300 Antibody

SKU : BHA10559762

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA790542
Alternative Names	E1A-associated protein p300; EP300; P300 transcription coactivator homolog;
Categories	Primary Antibodies
Clonality	polyclonal
Description	Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter.Wuchao Yuan, J. Biol. Chem., Apr 1996; 271: 9009. E. Tory Manning, Mol. Cell. Biol., Jun 2001; 21: 3876 - 3887. Jiwen Li, Mol. Cell. Biol., Mar 2000; 20: 2031 - 2042. Gopalswamy Jayaraman, J. Biol. Chem., Jun 1999; 274: 17342.
Host	Rabbit
Immunogen	Synthesized peptide derived from N-terminal of Human p300.
Involvement In Disease	Rubinstein-Taybi syndrome 2 (RSTS2)
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Wuchao Yuan, J. Biol. Chem., Apr 1996; 271: 9009. E. Tory Manning, Mol. Cell. Biol., Jun 2001; 21: 3876 - 3887. Jiwen Li, Mol. Cell. Biol., Mar 2000; 20: 2031 - 2042. Gopalswamy Jayaraman, J. Biol. Chem., Jun 1999; 274: 17342.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total p300 protein.
Subcellular Location	Cytoplasm, Nucleus, Chromosome

Uniprot	Q09472
Database Links	HGNC:3373 OMIM:602700 KEGG:hsa:2033 STRING:9606.ENSP00000263253 UniGene:Hs.517517
Function	Functions as histone acetyltransferase and regulates transcription via chromatin remodeling
Pathway	cAMP signaling pathway HIF-1 signaling pathway Jak-STAT signaling pathway Melanogenesis Notch signaling pathway TGF-beta signaling pathway Wnt signaling pathway Cell cycle FoxO signaling pathway Glucagon signaling pathway

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:500-1:3000
IHC	1:50-1:100

Tested Application

ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559762	CSB-PA790542	100ul	$297		Add to Cart

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