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Home / Primary Antibodies / IHC Antibodies

CCND1 Antibody

SKU : BHA10557199

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA004811KA01HU
Alternative Names	CCND1;BCL1;D11S287E;PRAD1;U21B31;G1/S-specific cyclin-D1;BCL-1 oncogene ;PRAD1 oncogene
Categories	Primary Antibodies
Description	Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point and pRb-dependent entry into S-phase. The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation.
Hgnc	1582
Host	Rabbit
Immunogen	A synthetic peptide of Human Cyclin D1
Intended Use	For research use only. Not for human, diagnostic or therapeutic use.
Involvement In Disease	Multiple myeloma (MM)
Isotype	IgG
Mgi	88313
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point and pRb-dependent entry into S-phase. The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation.
Size	100μl(100μg)
Species	Homo Sapiens (Human)
Subcellular Location	Nucleus, Cytoplasm, Membrane

Rgd	68384
Target Details	This protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Uniprot	P24385
Database Links	HGNC:1582 OMIM:168461 KEGG:hsa:595 STRING:9606.ENSP00000227507 UniGene:Hs.523852
Function	Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Pathway	Hedgehog signaling pathway Hippo signaling pathway Jak-STAT signaling pathway p53 signaling pathway PI3K-Akt signaling pathway Wnt signaling pathway Focal adhesion Cell cycle Cellular senescence AGE-RAGE signaling pathway in diabetic complications AMPK signaling Apelin signaling pathway FoxO signaling pathway
Protein Families	Cyclin family, Cyclin D subfamily
Research Area	Cell Cycle

Buffer	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Format	liquid
Purification	Affinity purification
Purity	Affinity purification
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage Buffer	Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Applications

ELISA, WB, IHC, ICC, IP

Description

ELISA ; WB ; IHC ; ICC ; IP, ICC ; IHC ; IP ; WB ; ELISA

Dilution

WB	1:500-1:1000
IHC	1:50-1:100
ICC	1:50-1:100
IP	1:20-1:50

Tested Application

ELISA,WB,IHC,ICC,IP;WB:1:500-1:1000,IHC:1:50-1:100,ICC:1:50-1:100,IP:1:20-1:50

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557199	CSB-PA004811KA01HU	100ul	$398		Add to Cart

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