AmCyan has been adapted from the corresponding full length cDNA for higher solubility brighter emission and rapid chromophore maturation (8?C12 hours). AmCyan1 has been human-codon optimized for enhanced translation in mammalian cells. AmCyan is a very good blue fluorescent reporter protein and useful in two-color analyses with DsRed2 DsRed-Express or AsRed2 and in three-color analyses with ZsYellow and HcRed.
Host
Mouse
Immunogen
The AmCyan Mouse Polyclonal Antibody detects AmCyan & AmCyan Tag protein.
Raised In
Mouse
Reactivity
Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep
Regulatory
RUO
Relevance
AmCyan has been adapted from the corresponding full length cDNA for higher solubility, brighter emission, and rapid chromophore maturation (8°C12 hours). AmCyan1 has been human-codon optimized for enhanced translation in mammalian cells. AmCyan is a very good blue fluorescent reporter protein, and useful in two-color analyses with DsRed2, DsRed-Express, or AsRed2, and in three-color analyses with ZsYellow and HcRed.
Buffer
pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.al
Form
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
Liquid
Purification
Antigen Affinity Purified
Purity
Antigen Affinity Purified
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
WB, IHC, IP, IF, ELISA
Description
WB ; IHC ; IP ; IF ; ELISA, IP ; WB ; IHC ; IF
Dilution
WB
1:500-1:2000
IHC
1:100-1:300
IP
2-5μg/mg
IF
1:200-1:1000
ELISA
1:20000
Tested Application
WB IHC IF IP Recommended dilution: Western blotting: 1:2000~1:5000,