Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2
Categories
Primary Antibodies
Cellular Localization
Nuclear, Cytoplasmic
Chromosome Location
10q21.2
Clonality
monoclonal
Description
Recognizes a 34kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.
Host
Mouse
Immunogen
C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli
Isotype
IgG2a Kappa
Positive Control
HeLa Cells or Tonsil
Reactivity
Human, Mouse, Rat, Guinea pig, Woodchuck, Xenopus, Chicken
Recombinant
FALSE
Regulatory
RUO
Swissprot
P06493
Gene Id
983
Clone No
A17.1.1
Symbol
CDC2
Weight
34kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
IFCLIP, WB, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IFCLIP ; WB ; IHC-F
Dilution
Immunofluorescence
1-2μg/ml
Immunoprecipitation
2-4μg/mg protein
Western Blotting
0.5-1.0μg/ml
Immunohistology (Formalin-fixed)
2-4μg/ml for 30 minutes at RT
No Description Available.
Reviews of Anti-undefined Antibody A17.1.1 Unconjugated BHA10208157