Anti-undefined Antibody LPSR,2385 Unconjugated BHA10208098

Mfr.No.	929-MSM5-P1ABX
Alternative Names	Lipopolysaccharide receptor ; LPS-R ; LPSR ; Mo2 ; Monocyte Differentiation Antigen 14 ; Myeloid cell-specific leucine-rich glycoprotein
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	5q31.1
Clonality	monoclonal
Description	Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
Host	Mouse
Immunogen	Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)
Isotype	IgG1 Kappa
Positive Control	THP-1 cells and human monocytes. Lymph nodes and tonsils.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P08571
Gene Id	929
Clone No	LPSR, 2385
Symbol	CD14
Weight	55kDa

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IHC-F

Dilution

Immunohistology (Formalin-fixed)

1-2μg/ml for 30 minutes at RT