Recognizes the -chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Host
Mouse
Immunogen
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Isotype
IgG1 Kappa
Positive Control
Human peripheral blood. Jurkat cells. Tonsil or lymph node.
Reactivity
Human, Chimpanzee
Recombinant
FALSE
Regulatory
RUO
Swissprot
P07766
Gene Id
916
Clone No
UCHT1
Symbol
CD3E
Weight
20kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
ELISA, FACS, IHC (fro)
Description
Order Ab without BSA, Optimal dilution for a specific application should be determined., ELISA ; FACS ; IHC (fro)
Dilution
ELISA
Use Ab at 2-4μg/ml for coating
Flow Cytometry
0.5-1μg/million cells
Immunohistology (Frozen)
1-2μg/ml for 30 minutes at RT
Reviews of Anti-undefined Antibody UCHT1 Unconjugated BHA10208121