Anti-Bromodeoxyuridine (BrdU) Antibody BHA10207142

Mfr.No.	MSM1-469-P1ABX
Alternative Names	Bromodeoxyuridine ; BUdr
Categories	Primary Antibodies
Cellular Localization	Nuclear
Clonality	Monoclonal
Description	It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Host	Mouse
Immunogen	Bromodeoxyuridine (BrdU) conjugated to KLH
Positive Control	Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU
Reactivity	All species
Recombinant	FALSE
Regulatory	RUO

Swissprot	Not Applicable
Uniprot	Not Applicable
Gene Id	Not Applicable

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC (Cat. #AP-9008)]Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
WB	0.5-1μg/ml for 30 minutes at RT
IHC-F	0.25-0.5μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207142	MSM1-469-P1ABX	100µg	$393.20		Add to Cart

Anti-Bromodeoxyuridine (BrdU) Antibody BHA10207142

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