Anti-Proximal Nephrogenic Antigen Antibody BHA10205888

Mfr.No.	768-MSM3-P0
Alternative Names	CA-IX ; CA9 ; CAIX ; Carbonate dehydratase IX ; Carbonic anhydrase 9 ; Carbonic anhydrase IX ; Carbonic dehydratase ; G250 ; Membrane antigen MN ; P54/58N
Categories	Primary Antibodies
Cellular Localization	Cell Surface, Cytoplasmic
Chromosome Location	9p13.3
Clonality	Monoclonal
Description	Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
Host	Mouse
Immunogen	Microsomal fraction of human renal cortical tissue homogenate
Positive Control	Normal kidney or renal cell carcinoma
Reactivity	Human, Horse
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q16790
Uniprot	63287
Gene Id	768

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml
WB	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10205888	768-MSM3-P0	20µg	$204.67		Add to Cart
BHA10205888	768-MSM3-P0	100µg	$393.20		Add to Cart

Anti-Proximal Nephrogenic Antigen Antibody BHA10205888

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