Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Host
Mouse
Immunogen
Recombinant human Rb1 protein
Isotype
IgG1 Kappa
Positive Control
HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.
Reactivity
Human, Mouse
Recombinant
FALSE
Regulatory
RUO
Swissprot
P06400
Uniprot
408528
Gene Id
5925
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
WB, IP, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., WB ; IP ; IHC-F
Dilution
WB
0.5-1μg/ml
IP
0.5-1 �g/500ug protein lysate
IHC-F
1-2μg/ml for 30 minutes at RT
No Description Available.
Reviews of Anti-Rb1 (Tumor Suppressor Protein) Antibody BHA10204645