Anti-MUC1 / EMA / CD227 Antibody BHA10203398

Mfr.No.	4582-MSM17-P1ABX
Alternative Names	Breast carcinoma-associated antigen DF3 ; CA15-3 ; Carcinoma-associated mucin Episialin ; Epithelial Membrane Antigen ; H23AG ; KL-6 ; MAM6 ; MUC-1 ; MUC-1/SEC ; MUC-1/X
Categories	Primary Antibodies
Cellular Localization	Cell Surface, Cytoplasmic
Chromosome Location	1q22
Clonality	Monoclonal
Description	This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.
Host	Mouse
Immunogen	Human milk-fat globule membranes (HMFGM)
Isotype	IgG1 Kappa
Positive Control	MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P15941
Uniprot	89603
Gene Id	4582

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-FP

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-FP

Dilution