Anti-MUC1 / EMA / CD227 Antibody BHA10203404

Mfr.No.	4582-MSM18-P1ABX
Alternative Names	Breast carcinoma-associated antigen DF3 ; CA15-3 ; Carcinoma-associated mucin Episialin ; Epithelial Membrane Antigen ; H23AG ; KL-6 ; MAM6 ; MUC-1 ; MUC-1/SEC ; MUC-1/X
Categories	Primary Antibodies
Cellular Localization	Cell Surface, Cytoplasmic
Chromosome Location	1q22
Clonality	Monoclonal
Description	In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Host	Mouse
Immunogen	Human milk-fat globule membranes (HMFGM)
Isotype	IgG2a Kappa
Positive Control	MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P15941
Uniprot	89603
Gene Id	4582

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution