Recognizes a protein of 20-25kDa, identified as human interferon. This MAb is specific to human IFN- and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN- (type I interferon).
Host
Mouse
Immunogen
Purified recombinant human IFN-gamma
Isotype
IgG2b Kappa
Positive Control
PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P01579
Uniprot
856
Gene Id
3458
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
IHC-F
1-2μg/ml for 30 min at RT
Reviews of Anti-Interferon gamma (IFNG) Antibody BHA10202084