Anti-AMACR / p504S Antibody BHA10201273

Mfr.No.	23600-RBP2-P0
Alternative Names	Alpha-methylacyl-CoA Racemase ; CBAS4 ; Da1-8 ; Macr1 ; RACE ; RM
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	5p13.3
Clonality	polyclonal
Description	This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and Ī �-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
Host	Rabbit
Immunogen	A synthetic peptide from human AMACR protein
Isotype	IgG
Positive Control	HEK cells or Prostate Adenocarcinoma
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q9UHK6
Uniprot	508343
Gene Id	23600

Buffer	200μg/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., IHC-F

Dilution

IHC-F

1:50-1:100 for 30-60 minutes at RT