Anti-Calponin-1 Antibody BHA10200561

Mfr.No.	1264-MSM3-P1ABX
Alternative Names	Calponin 1 basic smooth muscle ; Calponin H1 smooth muscle ; Calponin-1 ; CNN1 ; Cnn1 ; Sm Calp ; SMCC
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	19p13.2
Clonality	Monoclonal
Description	Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Host	Mouse
Immunogen	Recombinant full-length human CNN1 protein (CNN1/832); Crude human uterus extract (CALP)
Isotype	IgG
Positive Control	Myoepithelial cells in breast ducts or uterus
Reactivity	Human, Rat
Recombinant	FALSE
Regulatory	RUO

Swissprot	P51911
Uniprot	465929
Gene Id	1264

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F, IHC(fro)

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F ; IHC(fro)

Dilution