Anti-Granulocyte-Colony Stimulating Factor (G-CSF) Antibody BHA10200626

Mfr.No.	1440-MSM1X-P0
Alternative Names	Pluripoietin ; Filgrastim ; Lenograstim ; CSF3OS ; G-CSF ; chromosome 17 open reading frame 33 (C17orf33) ; colony stimulating factor 3 (granulocyte)
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	17q21.1
Clonality	Monoclonal
Description	This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73% sequence identity at the amino acid level.
Host	Mouse
Immunogen	Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes
Isotype	IgG1
Positive Control	HL60 cells. Tonsil or lymph node
Reactivity	Human, Macaque Monkey
Recombinant	FALSE
Regulatory	RUO

Swissprot	P09919
Uniprot	2233
Gene Id	1440

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF, IHC-F, IHC(fro)

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F ; IHC(fro)

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT
IHC(fro)

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SKU	Supplier No.	Size	Your price	Quantity
BHA10200626	1440-MSM1X-P0	20µg	$204.67		Add to Cart
BHA10200626	1440-MSM1X-P0	100µg	$393.20		Add to Cart

Anti-Granulocyte-Colony Stimulating Factor (G-CSF) Antibody BHA10200626

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