This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Host
Mouse
Immunogen
Purified GST-p27 fusion protein of human origin
Isotype
IgG1 Kappa
Positive Control
ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
Reactivity
Human, Mouse, Rat, Monkey
Recombinant
FALSE
Regulatory
RUO
Swissprot
P46527
Uniprot
238990
Gene Id
1027
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
WB
0.5-1μg/ml
IHC-F
0.25-0.5μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
No Description Available.
No Description Available.
Reviews of Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody BHA10200088