Human Von Hippel-lindau Disease Tumor Suppressor

Mfr.No.

E6401Hu

Sensitivity

7.03ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

HRCA1;Protein G7;pVHL;RCA1;VHL;VHL1;von Hippel-Lindau disease tumor suppressor

Molecular Weight

24153

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6401Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	786.84	48.37	6.15
Intra-Assay	2	18	249.49	14.81	5.94
Intra-Assay	3	18	98.74	5.53	5.6

Background

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.

Protocol

These standard curves of E6401Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.212	2.163	2.116
	2.113
800ng/L	1.717	1.633	1.587
	1.549
400ng/L	1.104	1.051	1.005
	0.999
200ng/L	0.644	0.659	0.613
	0.674
100ng/L	0.419	0.448	0.402
	0.476
0ng/L	0.029	0.046	0
	0.063

Gene ID	7428
UniProt ID	P40337

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107763	E6401Hu	48T	$320		Add to Cart
BHE12107763	E6401Hu	96T	$458		Add to Cart

Human Von Hippel-lindau Disease Tumor Suppressor - VHL ELISA Kit

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