Matrices listed below were spiked with certain level of VHL and the recovery rates were calculated by comparing the measured value to the expected amount of VHL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-101
93
EDTA plasma(n=5)
90-99
96
heparin plasma(n=5)
88-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VHL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
VHL ELISA Kit allows for the in vitro quantitative determination of VHL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human VHL(Von Hippel-Lindau disease tumor suppressor) ELISA Kit