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Home / ELISA Kit

Human Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit

SKU : BHE13702280

VEGF165 is an angiogenic cytokine that also regulates vascular permeability.The neuropilin-1 (np1) receptor binds the 165 amino-acid form of VEGF165 and functions as an enhancer that potentiates VEGF165 signaling via the VEGFR-2 tyrosine-kinase receptor. A VEGF165 mutant (VEGF165KF) that binds to neuropilins but displays a much lower affinity toward VEGFR-1 and VEGFR-2. VEGF165KF failed to induce VEGFR-2 phosphorylation in cells lacking neuropilins. However, in the presence of np1, VEGF165KF bound weakly to VEGFR-2, induced VEGFR-2 phosphorylation, and activated ERK1/2. Interestingly, VEGF165KF did not promote formation of VEGFR-2/np1 complexes nor did high concentrations of VEGF165KF inhibit VEGF165 induced formation of such complexes, suggesting that VEGF165 does not stabilize VEGFR-2/np1 complexes by forming bridges spanning VEGFR-2 and np1.

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Mfr.No.	KTE60061
Description	This Human Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF121.
Alternative Names	VEGF121
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Vascular Endothelial Growth Factor 121 microplate Human Vascular Endothelial Growth Factor 121 standard Human Vascular Endothelial Growth Factor 121 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit has high sensitivity and excellent specificity for detection of Human VEGF121. No significant cross-reactivity or interference between Human VEGF121 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	VEGF165 is an angiogenic cytokine that also regulates vascular permeability.The neuropilin-1 (np1) receptor binds the 165 amino-acid form of VEGF165 and functions as an enhancer that potentiates VEGF165 signaling via the VEGFR-2 tyrosine-kinase receptor. A VEGF165 mutant (VEGF165KF) that binds to neuropilins but displays a much lower affinity toward VEGFR-1 and VEGFR-2. VEGF165KF failed to induce VEGFR-2 phosphorylation in cells lacking neuropilins. However, in the presence of np1, VEGF165KF bound weakly to VEGFR-2, induced VEGFR-2 phosphorylation, and activated ERK1/2. Interestingly, VEGF165KF did not promote formation of VEGFR-2/np1 complexes nor did high concentrations of VEGF165KF inhibit VEGF165 induced formation of such complexes, suggesting that VEGF165 does not stabilize VEGFR-2/np1 complexes by forming bridges spanning VEGFR-2 and np1.
Notes	This Human Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF121 in samples. An antibody specific for VEGF121 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGF121 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF121 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF121 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Antigen	VEGF121
Tag	VEGF121
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF121 in samples. An antibody specific for VEGF121 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGF121 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF121 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF121 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
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