Human Tyrosine-protein Kinase Jak1

Mfr.No.

E4174Hu

Alternative Names

JAK 1;JAK1;JAK-1;Janus kinase 1;Tyrosine-protein kinase JAK1

Reactivity

Human

Detection Range

1-16ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

133277

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.53ng/mL

Background

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed: 7615558). Kinase partner for the interleukin (IL)-2 receptor (PubMed: 11909529) as well as interleukin (IL)-10 receptor (PubMed: 12133952).

Gene ID	3716
UniProt ID	P23$458.00\|$300.00
Antigen	JAK1

Research Area

Others

Protocol

These standard curves of E4174Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/mL	2.613	2.512	2.451
	2.411
8ng/mL	1.352	1.369	1.309
	1.387
4ng/mL	0.734	0.744	0.683
	0.755
2ng/mL	0.384	0.403	0.343
	0.423
1ng/mL	0.224	0.235	0.174
	0.245
0ng/mL	0.062	0.061	0
	0.06

Precision

We measured random samples of E4174Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.1	0.13	6.1
Intra-Assay	2	18	9.2	0.27	3
Intra-Assay	3	18	5.1	0.2	3.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105574	E4174Hu	48T	$320		Add to Cart
BHE12105574	E4174Hu	96T	$458		Add to Cart

Human Tyrosine-protein Kinase Jak1 - JAK1 ELISA Kit

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