Human TRIM25(E3 ubiquitin/ISG15 ligase TRIM25)ELISA Kit

Mfr.No.

EH13197

Description

Human TRIM25(E3 ubiquitin/ISG15 ligase TRIM25)ELISA Kit

Alternative Names

E3 ubiquitin/ISG15 ligase TRIM25 ELISA Kit, Estrogen-responsive finger protein ELISA Kit, RING finger protein 147 ELISA Kit, RING-type E3 ubiquitin transferase ELISA Kit, RING-type E3 ubiquitin transferase TRIM25 ELISA Kit, Tripartite motif-containing protein 25 ELISA Kit, Ubiquitin/ISG15-conjugating enzyme TRIM25 ELISA Kit, Zinc finger protein 147 ELISA Kit, EFP ELISA Kit, RNF147 ELISA Kit, ZNF147 ELISA Kit, TRIM25 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

31.25-2000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of TRIM25 and the recovery rates were calculated by comparing the measured value to the expected amount of TRIM25 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	95-101	99
EDTA plasma(n=5)	88-104	97
heparin plasma(n=5)	92-104	99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRIM25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	86-105%	90-102%	87-104%
EDTA plasma(n=5)	85-98%	84-101%	83-96%
heparin plasma(n=5)	81-93%	89-99%	81-99%

Sensitivity

< 18.75pg/ml

Notes

For Research Use Only

UniProt ID	Q14258

Applications	ELISA
Applications Notes	TRIM25 ELISA Kit allows for the in vitro quantitative determination of TRIM25 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10808023	EH13197	96T	$400		Add to Cart

Human TRIM25 (E3 ubiquitin/ISG15 ligase TRIM25)ELISA Kit

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