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Home / ELISA Kit

Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit

SKU : BHE13702896

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.
Sequence analysis predicted that the 346-amino acid SFRP4 protein contains an N-terminal signal peptide, no transmembrane domain, and a hydrophilic C terminus. In situ hybridization analysis demonstrated exclusive expression in stromal and myometrial cells, particularly in endometrium and breast. Upregulated expression was detected in endometrial and breast carcinomas.

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Mfr.No.	KTE60680
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP4.
Alternative Names	SFRP4; FRP-4; FRPHE; MGC26498; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4
Detection Method	Colorimetric
Kit Components	Human Secreted frizzled-related protein 4 microplate Human Secreted frizzled-related protein 4 standard Human Secreted frizzled-related protein 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFRP4. No significant cross-reactivity or interference between Human SFRP4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. Sequence analysis predicted that the 346-amino acid SFRP4 protein contains an N-terminal signal peptide, no transmembrane domain, and a hydrophilic C terminus. In situ hybridization analysis demonstrated exclusive expression in stromal and myometrial cells, particularly in endometrium and breast. Upregulated expression was detected in endometrial and breast carcinomas.
Notes	This Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP4 in samples. An antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFRP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6424

Applications	ELISA
Applications Notes	This Human Secreted frizzled-related protein 4 (SFRP4) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP4 in samples. An antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFRP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702896	KTE60680	48 T	$359		Add to Cart
BHE13702896	KTE60680	96 T	$619		Add to Cart
BHE13702896	KTE60680	96 Tx5	$2809		Add to Cart
BHE13702896	KTE60680	96 Tx50	$26209		Add to Cart

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