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Home / ELISA Kit

Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit

SKU : BHE13703012

RhoC (Ras homolog gene family, member C) is a small (~21 kDa) signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases.It cycles between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility.
It is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

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Mfr.No.	KTE60796
Description	This Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit employs a two-site sandwich ELISA to quantitate RHOC.
Alternative Names	RHOC; RP11-426L16.4; ARH9; ARHC; H9; MGC1448; MGC61427; RHOH9; OTTHUMP00000013802; OTTHUMP00000013805; OTTHUMP00000013807; RAS-related homolog 9; Rho-related GTP-binding protein RhoC; oncogene RHO H9; rhoC GTPa
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Rho-related GTP-binding protein RhoC microplate Human Rho-related GTP-binding protein RhoC standard Human Rho-related GTP-binding protein RhoC detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit has high sensitivity and excellent specificity for detection of Human RHOC. No significant cross-reactivity or interference between Human RHOC and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RhoC (Ras homolog gene family, member C) is a small (~21 kDa) signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases.It cycles between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. It is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Notes	This Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit employs a two-site sandwich ELISA to quantitate RHOC in samples. An antibody specific for RHOC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRHOC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHOC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHOC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	389
Antigen	RHOC
Tag	RHOC
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Rho-related GTP-binding protein RhoC (RHOC) ELISA Kit employs a two-site sandwich ELISA to quantitate RHOC in samples. An antibody specific for RHOC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRHOC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHOC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHOC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703012	KTE60796	48 T	$339		Add to Cart
BHE13703012	KTE60796	96 T	$589		Add to Cart
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BHE13703012	KTE60796	96 Tx50	$24569		Add to Cart

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