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Home / ELISA Kit

Human Ras-related protein M-Ras (MRAS) ELISA Kit

SKU : BHE13703758

MRAS encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for MRAS.

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Mfr.No.	KTE61543
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Ras-related protein M-Ras (MRAS) ELISA Kit employs a two-site sandwich ELISA to quantitate MRAS.
Alternative Names	MRAS; FLJ42964; M-RAs; R-RAS3; RRAS3; muscle and microspikes RAS
Detection Method	Colorimetric
Kit Components	Human Ras-related protein M-Ras microplate Human Ras-related protein M-Ras standard Human Ras-related protein M-Ras detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ras-related protein M-Ras (MRAS) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRAS. No significant cross-reactivity or interference between Human MRAS and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MRAS encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for MRAS.
Notes	This Human Ras-related protein M-Ras (MRAS) ELISA Kit employs a two-site sandwich ELISA to quantitate MRAS in samples. An antibody specific for MRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRAS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	22808

Applications	ELISA
Applications Notes	This Human Ras-related protein M-Ras (MRAS) ELISA Kit employs a two-site sandwich ELISA to quantitate MRAS in samples. An antibody specific for MRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRAS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703758	KTE61543	48 T	$339		Add to Cart
BHE13703758	KTE61543	96 T	$589		Add to Cart
BHE13703758	KTE61543	96 Tx5	$2629		Add to Cart
BHE13703758	KTE61543	96 Tx50	$24569		Add to Cart

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