Human Protein FADD(FADD) ELISA kit

Human Protein FADD(FADD) ELISA kit

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Mfr.No.

CSB-EL007958HU

Sensitivity

31.25 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Fas (TNFRSF6)-associated via death domain, GIG3, MGC8528, MORT1, Fas-associated via death domain, Fas-associating death domain-containing protein, Fas-associating protein with death domain, growth-inhibiting gene 3 protein, mediator of receptor-

Recovery

The recovery of rat MTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-108
EDTA plasma (n=4)	96	92-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-96
1:2	Average %	97
	Range %	91-105
1:4	Average %	98
	Range %	96-106
1:8	Average %	95
	Range %	89-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.725	2.905	2.815	2.614
1000	2.222	2.102	2.162	1.961
500	1.527	1.507	1.517	1.316
250	0.920	0.930	0.925	0.724
125	0.577	0.589	0.583	0.382
62.5	0.432	0.423	0.428	0.227
31.25	0.302	0.296	0.299	0.098
0	0.197	0.204	0.201	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q13158

Applications	ELISA
Research Area	FADD

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503974	CSB-EL007958HU	96T	$695	Add to Cart
BHE10503974	CSB-EL007958HU	5x96T	$3336	Add to Cart
BHE10503974	CSB-EL007958HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.