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Home / ELISA Kit

Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit

SKU : BHE13703510

Phosphoenolpyruvate Carboxykinase 2,Mitochondrial is a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver.
The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer.

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Mfr.No.	KTE61294
Description	This Human Phosphoenolpyruvate carboxykinase (GTP), mitochondrial (PCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCK2.
Alternative Names	PCK2; PEPCK; PEPCK-M; PEPCK2; PEP carboxykinase; mitochondrial phosphoenolpyruvate carboxykinase 2; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Phosphoenolpyruvate carboxykinase (GTP), mitochondrial microplate Human Phosphoenolpyruvate carboxykinase (GTP), mitochondrial standard Human Phosphoenolpyruvate carboxykinase (GTP), mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Phosphoenolpyruvate carboxykinase (GTP), mitochondrial (PCK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCK2. No significant cross-reactivity or interference between Human PCK2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Phosphoenolpyruvate Carboxykinase 2,Mitochondrial is a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer.
Notes	This Human Phosphoenolpyruvate carboxykinase (GTP), mitochondrial (PCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCK2 in samples. An antibody specific for PCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5106
Antigen	PCK2
Tag	PCK2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Phosphoenolpyruvate carboxykinase (GTP), mitochondrial (PCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCK2 in samples. An antibody specific for PCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703510	KTE61294	48 T	$339		Add to Cart
BHE13703510	KTE61294	96 T	$589		Add to Cart
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BHE13703510	KTE61294	96 Tx50	$24569		Add to Cart

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