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Home / ELISA Kit

Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit

SKU : BHE13703439

The PHACTR proteins share highest similarity in sequences surrounding the N- and C-terminal RPEL repeats and in the C-terminal actin- and PP1-binding domains. In situ hybridization of rat brain showed enrichment of Phactr1 in cerebral cortex, hippocampus, olfactory tubercle, nucleus accumbens, caudate-putamen, and piriform cortex.
Endogenous PP1 coprecipitated with ectopically expressed rat Phactr1 in human embryonic kidney cells. Phactr1 also coprecipitated with actin in rat brain lysates. Yeast 2-hybrid analysis of a series of Phactr1 C-terminal truncation mutants indicated that PP1 bound to Phactr1 near the C terminus, while actin bound in a region containing RPEL repeats 2 and 3.

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Mfr.No.	KTE61223
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PHACTR1.
Alternative Names	PHACTR1; RP1-257A7.1; KIAA1733; MGC126575; MGC126577; RPEL; RPEL1; dJ257A7.2; RPEL repeat containing 1
Detection Method	Colorimetric
Kit Components	Human Phosphatase and actin regulator 1 microplate Human Phosphatase and actin regulator 1 standard Human Phosphatase and actin regulator 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PHACTR1. No significant cross-reactivity or interference between Human PHACTR1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The PHACTR proteins share highest similarity in sequences surrounding the N- and C-terminal RPEL repeats and in the C-terminal actin- and PP1-binding domains. In situ hybridization of rat brain showed enrichment of Phactr1 in cerebral cortex, hippocampus, olfactory tubercle, nucleus accumbens, caudate-putamen, and piriform cortex. Endogenous PP1 coprecipitated with ectopically expressed rat Phactr1 in human embryonic kidney cells. Phactr1 also coprecipitated with actin in rat brain lysates. Yeast 2-hybrid analysis of a series of Phactr1 C-terminal truncation mutants indicated that PP1 bound to Phactr1 near the C terminus, while actin bound in a region containing RPEL repeats 2 and 3.
Notes	This Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PHACTR1 in samples. An antibody specific for PHACTR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHACTR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHACTR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHACTR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	221692

Applications	ELISA
Applications Notes	This Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PHACTR1 in samples. An antibody specific for PHACTR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHACTR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHACTR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHACTR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703439	KTE61223	48 T	$339		Add to Cart
BHE13703439	KTE61223	96 T	$589		Add to Cart
BHE13703439	KTE61223	96 Tx5	$2629		Add to Cart
BHE13703439	KTE61223	96 Tx50	$24569		Add to Cart

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