Human Nuclear Factor of Activated T-cells 5

Mfr.No.

E5792Hu

Alternative Names

NFAT 5;NFAT5;NF-AT5;Nuclear factor of activated T-cells 5;T-cell transcription factor NFAT5;TonE-binding protein;TonEBP;Tonicity-responsive enhancer-binding protein

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

165763

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.09ng/L

Background

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity (PubMed: 10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed: 22266867). Binds the DNA consensus sequence 5'-(ACT)(AG)TGGAAA(CAT)A(TA)(ATC)(CA)(ATG)(GT)(GAC)(CG)(CT)-3' (PubMed: 10377394).

Gene ID	10725
UniProt ID	O94916
Antigen	NFAT5

Research Area

Immunology

Protocol

These standard curves of E5792Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.65	2.581	2.536
	2.513
1200ng/L	1.472	1.431	1.386
	1.39
600ng/L	0.807	0.795	0.75
	0.784
300ng/L	0.517	0.525	0.48
	0.533
150ng/L	0.202	0.22	0.176
	0.239
0ng/L	0.043	0.045	0
	0.046

Precision

We measured random samples of E5792Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1207.8	22.18	1.8
Intra-Assay	2	18	997.2	52.04	5.2
Intra-Assay	3	18	519.4	28.42	5.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107154	E5792Hu	48T	$320		Add to Cart
BHE12107154	E5792Hu	96T	$458		Add to Cart

Human Nuclear Factor of Activated T-cells 5 - NFAT5 ELISA Kit

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